Regulatory

Part:BBa_K1163400:Design

Designed by: Emiel van der Kouwe   Group: iGEM13_Evry   (2013-09-22)


AceB promoter, iron sensitive, down-regulated by dimerized FUR protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 277
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Impossible to idengitfy the RBS and FUR binding site precisely


Source

Extracted form genomic sequence of E. coli MG1655

References